pGEV-LEU2 plasmid in E. coli (ATCC® MBA-172)

Depositors: J Pinkham

Permits and Restrictions

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Designations pGEV-LEU2 plasmid in E. coli
Depositors J Pinkham
Biosafety Level 1
Vector Information
Size (kb): 8.6600000000000000
Vector: pGEV-LEU2 plasmid in E. coli (plasmid)
Type: shuttle
Promoters: Promoter for expression MRP7
Construction: pRS315, MRP7, GAL4, Estradiol receptor-alpha, Herpes simplex virus protein 16
Comments
A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS314. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Tightly regulated, B-Estradiol Dose-Dependent Expression System for Yeast. BioTechniques 29: 1226-1231, 2000.

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.
Product Sheet
Product Sheet
Product Sheet