BK-4E (ATCC® 98045)

Organism: eukaryotic cells  /  Clone Type: Clone  /  Depositors: Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky

Permits and Restrictions

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Designations BK-4E
Species eukaryotic cells
Depositors Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky
Applications
in suitable host, produces protein eukaryotic translation initiation factor 4E
Vector
Construct size (kb): 12.80000019073486
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: BK shuttle
Intact vector size: 11.414
Type of vector: phagemid
Vector end: XhoI
Vector end: BamHI
Cloning sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Polylinker sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Construction: RDB-0
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: T/t-Ag, <-
replicon: BK papovavirus
marker(s): neoR, <-
promoter: SV40
enhancer: dioxin responsive cytochrome P-450
promoter for expression: MMTV, ->
promoter for in vitro transcription: SP6, ->
MCS: AatII/.../SacII, ->
promoter for in vitro transcription: T7, <-
replicon: f1, <-
marker(s): ampR, ->
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: eukaryotic cells
Gene symbol: EIF4E
Other gene symbols: EIF-4E
Gene name: eukaryotic translation initiation factor 4E
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: BamHI linker
Insert size (kb): 1.4
Cross references:
Insert lengths(kb): 1.399999976158142
Gene product: eukaryotic translation initiation factor 4E [EIF4E]
Target Gene: eukaryotic translation initiation factor 4E
Insert Size (kb) 1.400
Media LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.5, 1.4; BamHI--13.0; EcoRI--13.0; PstI--7.5, 3.8, 1.0; XhoI--13.0.
References

De Benedetti A, Rhoads RE. Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrrant growth and morphology. Proc. Natl. Acad. Sci. USA 87: 8212-8216, 1990. PubMed: 2122455