BK-4E (ATCC® 98045)

Organism: eukaryotic cells  /  Clone Type: Clone  /  Depositors: Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky

Permits and Restrictions

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Designations BK-4E
Species eukaryotic cells
Depositors Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky
Applications
in suitable host, produces protein eukaryotic translation initiation factor 4E
Vector
Construct size (kb): 12.80000019073486
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: BK shuttle
Intact vector size: 11.414
Type of vector: phagemid
Vector end: XhoI
Vector end: BamHI
Cloning sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Polylinker sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Construction: RDB-0
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: T/t-Ag, <-
replicon: BK papovavirus
marker(s): neoR, <-
promoter: SV40
enhancer: dioxin responsive cytochrome P-450
promoter for expression: MMTV, ->
promoter for in vitro transcription: SP6, ->
MCS: AatII/.../SacII, ->
promoter for in vitro transcription: T7, <-
replicon: f1, <-
marker(s): ampR, ->
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: eukaryotic cells
Gene symbol: EIF4E
Other gene symbols: EIF-4E
Gene name: eukaryotic translation initiation factor 4E
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: BamHI linker
Insert size (kb): 1.4
Cross references:
Insert lengths(kb): 1.399999976158142
Gene product: eukaryotic translation initiation factor 4E [EIF4E]
Target Gene: eukaryotic translation initiation factor 4E
Insert Size (kb) 1.400
Media LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.5, 1.4; BamHI--13.0; EcoRI--13.0; PstI--7.5, 3.8, 1.0; XhoI--13.0.
References

De Benedetti A, Rhoads RE. Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrrant growth and morphology. Proc. Natl. Acad. Sci. USA 87: 8212-8216, 1990. PubMed: 2122455

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Product Sheet