pRBBP1-III cDNA (ATCC® 95683)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pRBBP1-III cDNA
GenBank Number

S57162

Species Homo sapiens, human
Applications
contains sequence retinoblastoma-binding protein 1, isoform III
Vector
Construct size (kb): 6.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: BamHI
Vector end: HindIII
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBBP1
Gene name: retinoblastoma-binding protein 1, isoform III
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.8
Cross references: DNA Seq. Acc.: S57162
Insert lengths(kb): 3.799999952316284
Gene product: retinoblastoma-binding protein 1, isoform III [RBBP1]
Target Gene: retinoblastoma-binding protein 1, isoform III
Insert Size (kb) 3.800
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.7; BamHI/HindIII--3.8, 3.0; EcoRI-- 3.0, 2.6, 1.2; HindIII--6.7; PstI--4.4, 1.8, 0.46.
References

Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946

Fattaey AR, et al. Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 8: 3149-3156, 1993. PubMed: 8414517

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