pRBBP1-III cDNA (ATCC® 95683)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pRBBP1-III cDNA
GenBank Number

S57162

Species Homo sapiens, human
Applications
contains sequence retinoblastoma-binding protein 1, isoform III
Vector
Construct size (kb): 6.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: BamHI
Vector end: HindIII
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBBP1
Gene name: retinoblastoma-binding protein 1, isoform III
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.8
Cross references: DNA Seq. Acc.: S57162
Insert lengths(kb): 3.799999952316284
Gene product: retinoblastoma-binding protein 1, isoform III [RBBP1]
Target Gene: retinoblastoma-binding protein 1, isoform III
Insert Size (kb) 3.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.7; BamHI/HindIII--3.8, 3.0; EcoRI-- 3.0, 2.6, 1.2; HindIII--6.7; PstI--4.4, 1.8, 0.46.
References

Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946

Fattaey AR, et al. Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 8: 3149-3156, 1993. PubMed: 8414517