pSLH16 [transgene 2] (ATCC® 79013)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pSLH16 [transgene 2]
Species Homo sapiens, human
Applications
in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor
Vector
Construct size (kb): 17.29998970031738
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ18R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: NotI
Vector end: NotI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Construction: pUC18, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Type of DNA: genomic
Insert end: NotI
Insert end: NotI
Insert size (kb): 14.4
Cross references:
Insert lengths(kb): 14.39999961853027
Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR]
Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1
Target Gene: low density lipoprotein receptor (familial hypercholesterolemia)
Insert Size (kb) 14.400
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): SalI--14.8, 2.5; EcoRI--12.8, 4.5; BglII--14.0, 3.3; SstI--17.3; NotI--14.4; 2.9.
The insert includes the following restriction sites: SalI (intron 1, 2.0 kb from the 5' NotI site); BglII (intron 3); EcoRI (cDNA segment).
Encodes the normal sequence.
The expected size of the correctly processed mRNA is 2.9 kb.
The NotI fragment consists of the following elements: the mouse metallothionein promoter I (1.7 kb); exon 1 of LDLR, containing a SstII site; intron 1 truncated by deletion of a RsaI (77 nt 3' to exon 1) / EcoRI (3.1 kb 5' to exon 2) fragment;
exons and introns 2-4; a cDNA fragment corresponding to exons 5-18; and transcription termination and polyadenylation signals from the human growth hormone receptor gene (0.6 kb).
References

Pathak RK, et al. Tissue-specific sorting of the human LDL receptor in polarized epithelia of transgenic mice. J. Cell Biol. 111: 347-359, 1990. PubMed: 2199454

Masayuki Yokode, personal communication

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