BSp49 [XLBSp49 MD] (ATCC® 68673)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: GJ Nabel

Permits and Restrictions

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Designations BSp49 [XLBSp49 MD]
GenBank Number

X61498

Species Homo sapiens, human
Depositors GJ Nabel
Applications
A 1.069 kb BstXI fragment lacking both the 5'-untranslated sequence and the repetitive region from the 3'-end of the clone has been used as a single copy probe.
For this construct, use the HindIII/BstXI fragment.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: BJAB cell line (B-lymphoid leukemia)
Gene product: nuclear factor of kappa light chain gene enhancer in B-Cells 2 (p100)(nuclear factor of kappa light chain gene enhancer in B-Cells 2 (p49)) [NFKB2]
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.9, 1.5; BamHI--4.4; HindIII--4.4; PstI--4.4; EcoRI--4.4.
The insert contains the following restriction sites (nt from the 5' end): BglII--875; PvuII--112; BstXI--1078; StuI--823, 1033.
Detects human genomic restriction fragments of the following approximate sizes (kb): EcoRI--12; BamHI--8.
A 1.069 kb BstXI fragment lacking both the 5'-untranslated sequence and the repetitive region from the 3'-end of the clone has been used as a single copy probe. For this construct, use the HindIII/BstXI fragment.
The insert is in the KS orientation (5' to 3').
A consensus Kozak sequence with a HindIII site was inserted at the initiation ATG and 5' untranslated sequences were deleted.
References

Schmid RM, et al. Cloning of an NK-kappaB subunit which stimulates HIV transcription in synergy with p65. Nature 352: 733-736, 1991. PubMed: 1876189

J P Cairns, personal communication

Gary J Nabel, personal communication

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