BSp49 [XLBSp49 MD] (ATCC® 68673)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: GJ Nabel

Permits and Restrictions

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Designations BSp49 [XLBSp49 MD]
GenBank Number

X61498

Species Homo sapiens, human
Depositors GJ Nabel
Applications
A 1.069 kb BstXI fragment lacking both the 5'-untranslated sequence and the repetitive region from the 3'-end of the clone has been used as a single copy probe.
For this construct, use the HindIII/BstXI fragment.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: BJAB cell line (B-lymphoid leukemia)
Gene product: nuclear factor of kappa light chain gene enhancer in B-Cells 2 (p100)(nuclear factor of kappa light chain gene enhancer in B-Cells 2 (p49)) [NFKB2]
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.9, 1.5; BamHI--4.4; HindIII--4.4; PstI--4.4; EcoRI--4.4.
The insert contains the following restriction sites (nt from the 5' end): BglII--875; PvuII--112; BstXI--1078; StuI--823, 1033.
Detects human genomic restriction fragments of the following approximate sizes (kb): EcoRI--12; BamHI--8.
A 1.069 kb BstXI fragment lacking both the 5'-untranslated sequence and the repetitive region from the 3'-end of the clone has been used as a single copy probe. For this construct, use the HindIII/BstXI fragment.
The insert is in the KS orientation (5' to 3').
A consensus Kozak sequence with a HindIII site was inserted at the initiation ATG and 5' untranslated sequences were deleted.
References

Schmid RM, et al. Cloning of an NK-kappaB subunit which stimulates HIV transcription in synergy with p65. Nature 352: 733-736, 1991. PubMed: 1876189

J P Cairns, personal communication

Gary J Nabel, personal communication