pCLL2213 (ATCC® 68362)

Organism: Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers  /  Clone Type: Clone  /  Depositors: American Cyanamid Co., BA Rasmussen, American Cyanamid Co.

Permits and Restrictions

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Designations pCLL2213
Species Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers
Depositors American Cyanamid Co., BA Rasmussen, American Cyanamid Co.
Vector
Construct size (kb): 13.19999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.600000381469727
Gene product: metallo-beta-lactamase [ccrA]
Insert Size (kb) 9.600
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 3.3; HindIII--4.2, 2.8, 2.6, 1.8, 1.35; KpnI--13.0.
The insert contains the following restriction sites (approximate kb from the end upstream of ccrA): HindIII--4.3, KpnI--4.8. The latter site interrupts the gene.
The vector is a derivative of pMK16 deleted for the carboxy terminal half of the tetR marker and has had the small EcoRI/BamHI fragment replaced with the MCS-containing EcoRI/BamHI fragment from plink 322.
References

Rasmussen BA, et al. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA. Mol. Microbiol. 5: 1211-1219, 1991. PubMed: 1956298

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