BSB- (ATCC® 67725)

Applications: vector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNA  /  Depositors: SC Pruitt

Permits and Restrictions

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Designations BSB-
Depositors SC Pruitt
Biosafety Level 1
Vector Information
Size (kb): 3.2100000381469730
Vector: BSB- (phagemid)
Promoters: Promoter T3
Construction: pBS(-), oligo BstXI adapter
Marker(s):ampR
Construct size (kb): 3.210000038146973
Features: marker(s): ampR
promoter: T3, T7
replicon: pMB1, f1
terminator: none
enhancer: none
Applications
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.45, 0.75; BamHI--3.2; NdeI--3.2; PvuII--2.65 + smaller; PstI--uncut.
A plasmid vector containing convenient priming sites for sequence analysis and synthesis of RNA in either direction from T3 and T7 promoters.
Constructed from Bluescribe M13- by insertion of a synthetic BstXI adapter between the PstI and XbaI sites of the polylinker region.
Contains the intergenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert.
BSB+ (away from cloning site) and BSB- (toward cloning site) differ in their orientation of the intergenic region from bacteriophage f1.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Pruitt SC. Expression vectors permitting cDNA cloning and enrichment for specific sequences by hybridization/selection. Gene 66: 121-134, 1988. PubMed: 2843427

Shipped freeze-dried
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