pGMC (ATCC® 65103)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Designations pGMC
GenBank Number

X52259

Species Homo sapiens, human
Vector
Construct size (kb): 3.160000085830689
Insert
DNA: genomic
Insert lengths(kb): 0.4199999868869782
Tissue: DNA from NIH 3T3 cells transformed by MCF7 DNA
Gene product: sec oncogene [SEC*]
Insert Size (kb) 0.420
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HincII/AccI--uncut; PvuII--2.35, 0.8; HindIII--3.1; EcoRI--3.1; EcoRI/HindIII--2.6, 0.5.
The insert extends from the PvuII site at position 41 of the published sequence to the AccI site at 462. Coding sequences extend from 21 to 347.
The insert is oriented such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand.
References

Lane MA, et al. Nucleotide sequence of a human oncogene active in tumors of secretory epithelium. Nucleic Acids Res. 18: 3068, 1990. PubMed: 2349114

Lane MA, et al. Activation of related transforming genes in mouse and human mammary carcinomas. Proc. Natl. Acad. Sci. USA 78: 5185-5189, 1981. PubMed: 6272293

Mary Ann L Lane, personal communication

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