8AC (ATCC® 63167)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: T Yorifuji, AL Beaudet

Permits and Restrictions

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Designations 8AC
GenBank Number

M60493

Species Mus musculus, mouse
Depositors T Yorifuji, AL Beaudet
Vector
Construct size (kb): 6.820000171661377
Insert
DNA: cDNA
Insert lengths(kb): 3.880000114440918
Gene product: cystic fibrosis transmembrane conductance regulator [Cftr]
Insert Size (kb) 3.880
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.5, 0.65; EcoRI--5.4, 1.45; HindIII--3.2, 2.3, 0.85, 0.7; PstI--6.9; PstI/XhoI--3.9, 2.9.
The insert contains the following restriction sites (nt from the 5' end): HindIII--85, 856, 1539, 3792; SalI--568; BamHI--1600, 2252; EcoRI--1510.
The insert extends from nt 2438-6304 of the reported sequence, including exons 13 through the 3' end. Also includes a short (14 nt) poly(A) tail not in the sequence record.
Transcription from the T3 promoter gives the sense strand.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Oliva D, et al. Complete structure of the human gene encoding neuron-specific enolase. Genomics 10: 157-165, 1991. PubMed: 2045099

Tohru Yorifuji, personal communication

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