pMCT35.1 [MCT35.1, pMCT35] (ATCC® 61008)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Permits and Restrictions

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Designations pMCT35.1 [MCT35.1, pMCT35]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
Codominant segregation was observed with the MspI RFLP in 55 three-generation families.
RsaI also identifies a polymorphism, but this observation has not been verified by family studies.
Otherwise, RFLPs were observed under normal hybridization and washing conditions.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.400000095367432
Gene product: DNA Segment, single copy [D17S31]
Alleles: A1, A2
Insert Size (kb) 2.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 2.4; EcoRI--5.0; HindIII--5.0; PstI--3.15, 1.65; BamHI--3.5, 1.65.
Codominant segregation was observed with the MspI RFLP in 55 three-generation families.
RsaI also identifies a polymorphism, but this observation has not been verified by family studies.
This probe was pre-associated with excess human DNA prior to hybridization. Otherwise, RFLPs were observed under normal hybridization and washing conditions.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII and TaqI.
References

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence pMCT35.1 on chromosome 17p [D17S31]. Nucleic Acids Res. 16: 783, 1988. PubMed: 2893351

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