pMCT35.1 [MCT35.1, pMCT35] (ATCC® 61008)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Permits and Restrictions

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Designations pMCT35.1 [MCT35.1, pMCT35]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
Codominant segregation was observed with the MspI RFLP in 55 three-generation families.
RsaI also identifies a polymorphism, but this observation has not been verified by family studies.
Otherwise, RFLPs were observed under normal hybridization and washing conditions.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.400000095367432
Gene product: DNA Segment, single copy [D17S31]
Alleles: A1, A2
Insert Size (kb) 2.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 2.4; EcoRI--5.0; HindIII--5.0; PstI--3.15, 1.65; BamHI--3.5, 1.65.
Codominant segregation was observed with the MspI RFLP in 55 three-generation families.
RsaI also identifies a polymorphism, but this observation has not been verified by family studies.
This probe was pre-associated with excess human DNA prior to hybridization. Otherwise, RFLPs were observed under normal hybridization and washing conditions.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII and TaqI.
References

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence pMCT35.1 on chromosome 17p [D17S31]. Nucleic Acids Res. 16: 783, 1988. PubMed: 2893351

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