ASSg3 (ATCC® 59880)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Northrup, WE O'Brien

Permits and Restrictions
Permits and Restrictions

View Permits

Designations ASSg3
Species Homo sapiens, human
Depositors H Northrup, WE O'Brien
Vector
Construct size (kb): 8.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 4.400000095367432
Tissue: fetal liver
Gene product: argininosuccinate synthetase(arginosuccinate synthetase 1) [ASS]
Alleles: A1, A1, A1, A2, A2, A2
Insert Size (kb) 4.400
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
When excised from pBR322 with EcoRI + HindIII, insert and vector are about the same size.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4; EcoRI--10.0; BamHI--10.0; BamHI/EcoRI--5.0, 4.0; PvuII--4.4.
Insert contains exons 11 and 12 as well as some intron sequence of the argininosuccinate synthetase gene.
The population studied was CEPH (Centre d'Etude du Polymorphisme Humain) families.
Enzyme(s) not detecting polymorphism: EcoRV, KpnI, ScaI, SphI, SstI, BanI, BclI, BglI, BglII, EcoRI, HincII, BanII, Fnu4HI, MboI, RsaI, TaqI, HindIII, PssI, PvuII, StuI, XbaI.
References

Northrup H, et al. Multilocus linkage analysis with the human argininosuccinate synthetase gene. Genomics 5: 442-444, 1989. PubMed: 2575581

Hope Northrup, personal communication

Product Sheet
Product Sheet
Product Sheet