pT7L-21 (ATCC® 40291)

Organism: Tetrahymena thermophila Nanney and McCoy  /  Clone Type: Clone  /  Depositors: Univ. Colorado, TR Cech, Univ. Colorado

Permits and Restrictions
Permits and Restrictions

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Designations pT7L-21
Species Tetrahymena thermophila Nanney and McCoy
Depositors Univ. Colorado, TR Cech, Univ. Colorado
Applications
After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme.
Vector
Construct size (kb): 3.200000047683716
Insert
DNA: genomic
Insert lengths(kb): 0.5
Gene product: ribozyme having endoribonuclease activity toward recognition sequence CUCU (RNA, ribosomal)
Insert Size (kb) 0.5
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.5; EcoRI--3.2; HindIII--3.2.
Distributed in aliquots of 200 ng dried.
Constructed by inserting the SphI/HindIII fragment [intervening sequence (IVS) minus the first 44 bp] from pBGST7 (ATCC 40288) into pUC18, and adding a synthetic EcoRI/SphI fragment containing a T7 promoter and bp 22-44 of the IVS.
After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme.
Classification KINGDOM: Protozoa
References

Cech TR, et al. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods. US Patent 4,987,071 dated Jan 22 1991

Product Sheet
Product Sheet
Product Sheet