DR-beta-B [E.coli HB101(pBR322(Pst)/HLA-DR-beta-B)] (ATCC® 39163)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Biogen N.V., JF Haley, Biogen N.V.

Permits and Restrictions

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Designations DR-beta-B [E.coli HB101(pBR322(Pst)/HLA-DR-beta-B)]
GenBank Number

A06805

Species Homo sapiens, human
Depositors Biogen N.V., JF Haley, Biogen N.V.
Vector
Construct size (kb): 5.578000068664551
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI Modification: dG tailed
Vector end: PstI Modification: dG tailed
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: HLA-DRB*
Gene name: major histocompatibility complex, class II, DR beta 1
(GB:A06805)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.3
Tissue: cell line IBW9 (beta lymphoblasts)
Type of DNA: cDNA
Insert end: Modification: dC tailed
Insert end: Modification: dC tailed
Insert size (kb): 1.215
Cross references: DNA Seq. Acc.: A06805
Nucleotides 1-1215 of the insert correspond to
nucleotides 1-1215 of A06805.
Insert lengths(kb): 1.2150000333786
Tissue: cell line IBW9 (beta lymphoblasts)
Gene product: major histocompatibility complex, class II, DR beta 1 (GB:A06805) [HLA-DRB*]
Insert Size (kb) 1.215
Media Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.6, 0.5; SstI--5.5; StuI--5.5.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.13; ScaI--0.32; PstI--0.38, 0.47, 0.99; XmnI--0.51, 0.63, 0.92; StuI--0.58.
References

Mach BF, et al. DNA sequences coding for the DR-beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto. US Patent 5,169,941 dated Dec 8 1992

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