c9395 (ATCC® 71258)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: L Riles

Permits and Restrictions

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Designations c9395
GenBank Number

Z46727

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors L Riles
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: SEC7
Gene name: protein transport protein
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: SUP35
Gene name: omnipotent suppressor protein 2
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: ARGR3
Gene name: arginine metabolism regulation
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: NGG1
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: UBC1
Gene name: ubiquitin-conjugating enzyme
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: SDH4
Gene name: succinate dehydrogenase membrane anchor subunit
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: CDC1
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: TCP20
Gene name: TriC chaperonin
Contains complete coding sequence?: Y
Chromosome: IV
Genome: Saccharomyces cerevisiae
Gene symbol: SLY1
Contains complete coding sequence?: Y
Chromosome: IV
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: DNA Seq. Acc.: Z46727
Gene product: protein transport protein [CDC1]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet