c9218 [9218] (ATCC® 70893)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Permits and Restrictions

View Permits

Designations c9218 [9218]
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Construct size (kb): 0.0
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
DNA: genomic
Genome: Saccharomyces cerevisiae
Gene symbol: MAK16
Contains complete coding sequence?: U
Chromosome: I; Localization: I L, 40 cM from CEN
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references:
Gene product: [MAK16]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 2.587, 3.039, 2.677, 1.558, 8.117, 1.363, 4.928, 2.186, 1.342, 4.237.
Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 1.4, 1.6, 2.3, 2.8, 3.3, 4.4, 5.3, 8.9.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70449, 70515.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Product Sheet
Product Sheet
Product Sheet