c9115 [9115] (ATCC® 70874)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Designations c9115 [9115]
GenBank Number

U18916

U18917

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Applications
The ATCC encourages users of this material to communicate their results to us.
Vector
Construct size (kb): 0.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: GLC7
Gene name: protein phosphatase type I
Contains complete coding sequence?: U
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GDI1
Gene name: GDP dissociation inhibitor
Contains complete coding sequence?: U
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: MAG1
Gene name: 3-methyladenine DNA glycosylase
Contains complete coding sequence?: U
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: UBP5
Gene name: ubiquitin-specific protease
Contains complete coding sequence?: U
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: DNA Seq. Acc.: U18916
Nucleotides 1-13368 of the insert correspond to
nucleotides 54936-68303 of U18916.
DNA Seq. Acc.: U18917
Nucleotides ? of the insert correspond to
nucleotides 1-23020 of U18917.
Gene product: protein phosphatase, type 1(protein phosphatase type I) [GDI1]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.251, 0.935, 1.917, 1.821, 1.805, 3.357, 0.812, 0.435, 1.845, 3.368, 5.251, 5.171, 0.997, 0.952, 0.637, 0.469, 2.283, 1.448, 1.004, 0.934.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70132, 70151, 70363, 70459, 70469.
NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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