Dictyostelium discoideum Raper (ATCC® 201386)

Strain Designations: AX4  /  Product Format: frozen

Permits and Restrictions

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Strain Designations AX4
Application
transformation host
Biosafety Level 1
Product Format frozen
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
It was recently found this strain can no longer be maintained and provided as a axenic culture. So it contains its food source bacteria.
Genome sequencing strain (Baylor College of Medicine, USA).
Medium ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions
Temperature: 25.0°C
Duration: This culture contains food source bacteria, and thus it is not axenic.
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor WF Loomis
Special Collection eumycetozoan
Chain of Custody
ATCC <<--WF Loomis<<--W. Nellen
Isolation
derived from strain AX3, ATCC 28368
Cross References

Complete Genome (GenBank) : AAFI00000000 Dictyostelium discoideum AX4, whole genome shotgun sequencing project

References

Chaumont F, et al. Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities. Proc. Natl. Acad. Sci. USA 94: 6202-6209, 1997. PubMed: 9177195

Knecht DA, et al. Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors. Mol. Cell. Biol. 6: 3973-3983, 1986. PubMed: 3025622

Eichinger L, et al. The genome of the social amoeba Dictyostelium discoideum. Nature 435: 43-57, 2005. PubMed: 15875012

Glöckner G, et al. Sequence and analysis of chromosome 2 of Dictyostelium discoideum. Nature 418: 79-85, 2002. PubMed: 12097910

Kuspa A, Loomis WF. Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome. Proc. Natl. Acad. Sci. USA 93: 5562-5556, 1996. PubMed: 8643615

Basic Documentation
Restrictions

Part of Eumycetozoan Project