A6 [A-6] (ATCC® CRL-8192)

Organism: Homo sapiens; Mus musculus, human; mouse  /  Cell Type: Hybridoma  / 

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Organism Homo sapiens; Mus musculus, human; mouse
Cell Type Hybridoma
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 1
Applications
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
The cells are useful as fusion partners for human B cells in the generation of human hybridomas.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
Comments
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
G-418 resistance was introduced to the human myeloma by transfection with pSV-2 neo r.
The cells are useful as fusion partners for human B cells in the generation of human hybridomas.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 x 10(5) and 1 x 10(6) cells/ml. Scrape attached cells if necessary.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: 75% CompleteGrowth Medium + 20% FBS + 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor H Kaplan
References

Kaplan HS, et al. Methods and cell lines for immortalization and monoclonal antibody production by antigen-stimulated B-lymphocytes. US Patent 4,574,116 dated Mar 4 1986

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kaplan HS, et al. Methods and cell lines for immortalization and monoclonal antibody production by antigen-stimulated B-lymphocytes. US Patent 4,574,116 dated Mar 4 1986