1A2 (ATCC® CRL-8119)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transforme  /  Disease: Lymphoma

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Organism Homo sapiens, human
Cell Type lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 CELLS CONTAIN HERPESVIRUS
Disease Lymphoma
Applications
This line was derived from the GM1500 lymphoma line.
The cells are resistant to 6-thioguanine.
The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from the GM1500 lymphoma line.
Genes Expressed
Epstein-Barr virus (EBV)
Comments
This line was derived from the GM1500 lymphoma line.
The cells are resistant to 6-thioguanine.
The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.
Complete Growth Medium RPMI 1640 medium with 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Interval: every 3 days
Medium Renewal: Every 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time 24 hrs (when cells are in logarithmic growth)
Name of Depositor Genetic Systems Corp.
References

Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984

Croce CM, et al. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984

Croce CM, et al. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999