MA-104 Clone 1 (ATCC® CRL-2378.1)

Organism: Cercopithecus aethiops  /  Cell Type: epithelial  /  Tissue: kidney  / 

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Organism Cercopithecus aethiops
Tissue kidney
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue.
Virus Susceptibility Simian rotavirus
Comments
This item is not available for international distribution.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:3 to 1:10
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Population Doubling Time 27 hours
Name of Depositor MM Vincent
Year of Origin 2001
References

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530

MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530

MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1).