M1/9.3.4.HL.2 (ATCC® TIB-122)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits View Restrictions

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells.
It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells. It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation. Tested and found negative for ectromelia virus (mousepox).
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse common leukocyte antigen (200000 dalton, CD45)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse common leukocyte antigen (200000 dalton, CD45)
Comments
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells. It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype rat IgG2a
Name of Depositor TA Springer
References

Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133

Stern PL, et al. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell 14: 775-783, 1978. PubMed: 567532

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058

Luqman M, et al. Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones. Eur. J. Immunol. 21: 17-22, 1991. PubMed: 1671357

Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980

Milstein C, et al. Monoclonal antibodies and cell surface antigens. Cell Biol. Int. Rep. 3: 1-16, 1979. PubMed: 88272

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):

References

Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133

Stern PL, et al. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell 14: 775-783, 1978. PubMed: 567532

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058

Luqman M, et al. Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones. Eur. J. Immunol. 21: 17-22, 1991. PubMed: 1671357

Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980

Milstein C, et al. Monoclonal antibodies and cell surface antigens. Cell Biol. Int. Rep. 3: 1-16, 1979. PubMed: 88272