Cl. Ly1+2-/9 (ATCC® CRL-8179)

Organism: Mus musculus, mouse  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain C57BL/6-TL+
Applications
This is a cloned T cell line derived from C57BL/6TL+ spleen cells.
The cells produce IL-3, TCGF2, MCGF2 and BSF-1.
Derivation
This is a cloned T cell line derived from C57BL/6TL+ spleen cells.
Genes Expressed
interleukin-3 (interleukin 3, IL-3); T cell growth factor 2 (TCGF2); mast cell growth factor 2 (MCGF2); B cell stimulating factor 1 (BSF-1)
Comments
This is a cloned T cell line derived from C57BL/6TL+ spleen cells.
The cells produce IL-3, TCGF2, MCGF2 and BSF-1.
Complete Growth Medium RPMI 1640 with 2.0 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; fetal bovine serum, 10%; rat T-STIM with Con A, 10%. Rat T-STIM with Con A can be obtained from Becton Dickinson, Catalogue No. 354115
Subculturing
Medium Renewal: Every 2 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 0.5 X 10 exp5 and 3 X 10 exp6 cells/ml.
Cryopreservation
RPMI 1640 medium 75%; fetal bovine serum, 20%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Dana Farber Cancer Inst.
References

Cantor HI, Nabel G. Lymphocyte growth factor. US Patent 4,613,459 dated Sep 23 1986

Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154

Nabel G, et al. Multiple biologic activities of a cloned inducer T-cell population. Proc. Natl. Acad. Sci. USA 78: 1157-1161, 1981. PubMed: 6785749

Mosmann TR, et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136: 2348-2357, 1986. PubMed: 2419430

Cross References

Nucleotide (GenBank) : E00420 cDNA sequence encoding peptide having MCGF and multi-CSF activity.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cantor HI, Nabel G. Lymphocyte growth factor. US Patent 4,613,459 dated Sep 23 1986

Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154

Nabel G, et al. Multiple biologic activities of a cloned inducer T-cell population. Proc. Natl. Acad. Sci. USA 78: 1157-1161, 1981. PubMed: 6785749

Mosmann TR, et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136: 2348-2357, 1986. PubMed: 2419430