CBRN1/6 (ATCC® CRL-2860)

Organism: Mus musculus, mouse  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain BALB/c
Applications
[Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. [Pubmed: 9614126].
Comments
Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. [Pubmed: 9614126].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG
Name of Depositor TA Springer
Year of Origin April 5, 1994
References

Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126

Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388

Basic Documentation
Other Documentation
Restrictions

Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s):

References

Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126

Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388