Malme-3 (ATCC® HTB-102)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue
skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 43 years
Gender male
Ethnicity Caucasian
Applications This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived.

Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology.
Derivation
This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived.
Clinical Data
43 years adult
Caucasian
male

Antigen Expression
HLA A2, Aw30, B13, B40(+/-), DRw7
Genes Expressed
HLA A2, Aw30, B13, B40(+/-), DRw7
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Interval: Subculture every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,13
D16S539: 9,12
D5S818: 11,13
D7S820: 9,12
THO1: 7,8
TPOX: 8,9
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor J Fogh
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.