CHL-1 (ATCC® CRL-9446)

Organism: Homo sapiens, human  /  Cell Type: melanoma  /  Tissue: skin  /  Disease: melanoma

Organism Homo sapiens, human
Tissue skin
Cell Type melanoma
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease melanoma
Applications
Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 107 cells/mL.
Storage Conditions liquid nitrogen vapor temperature
Derivation
This is a derivative of the RPMI 7932 human melanoma cell line (Bowes).
Genes Expressed
human tissue plasminogen activator (t-PA)
Cellular Products
human tissue plasminogen activator (t-PA)
Comments
This cell line produces about 0.1 mU of t-PA per cell per day.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 8,11
D16S539: 13
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
Name of Depositor Berlex Laboratories, Inc.
References

Cashion L, et al. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991

Basic Documentation
References

Cashion L, et al. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991