EPC [Epithelioma Papulosum Cyprini] (ATCC® CRL-2872)

Organism: Fathead minnow  /  Cell Type: epithelial  /  Tissue: skin  / 

Organism Fathead minnow
Tissue skin
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Applications
The EPC line has a broad sensitivity for fish viruses. It is used internationally for isolation, propagation and diagnostic assays for fish viruses.
Storage Conditions liquid nitrogen vapor phase
Images
Virus Susceptibility Infectious pancreatic necrosis virus
Infectious hematopoietic necrosis virus
Spring Viremia of Carp Virus
Viral hemorrhagic septicemia virus
Epizootic hematopoietic necrosis virus
Largemouth bass virus
Comments
ATCC CRL-2872, originally deposited as Carp (Cyprinus carpio), was subsequently found to be Fathead Minnow (Pimephales promelas) through cytochrome c oxidase subunit I testing at ATCC.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.
  6. Incubate cultures at 25°C. We recommend that you maintain cultures at a cell concentration between 1 x 105 and 2 x 105 cells/cm2.

Subcultivation Ratio: 1:4 to 1:6
Cryopreservation
Freeze medium: culture medium, 95%; DMSO,5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 25°C
Max Temperature: 30°C
Min Temperature: 20°C
Population Doubling Time about 36 hours
Name of Depositor J Winton
Year of Origin 1969
References

Fijan N, et al. Some properties of the Epithelioma Papulosum Cyprini (EPC) cell line from carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134 E: 207-220, 1983.

Fryer JL, Lannan CN. Three decades of fish cell culture: A current listing of cell lines derived from fishes. J. Tissue Culture Methods 16: 87-94, 1994.

Lorenzen E, et al. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis Aquat Org 37: 81-88, 1999. PubMed: 10494498

Loh PC, et al. Growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. J. Virol. Methods 28: 273-280, 1990. PubMed: 2117021

Basic Documentation
Other Documentation
References

Fijan N, et al. Some properties of the Epithelioma Papulosum Cyprini (EPC) cell line from carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134 E: 207-220, 1983.

Fryer JL, Lannan CN. Three decades of fish cell culture: A current listing of cell lines derived from fishes. J. Tissue Culture Methods 16: 87-94, 1994.

Lorenzen E, et al. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis Aquat Org 37: 81-88, 1999. PubMed: 10494498

Loh PC, et al. Growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. J. Virol. Methods 28: 273-280, 1990. PubMed: 2117021