GS-109-V-63 [G96] (ATCC® CRL-1614)

Organism: Homo sapiens, human  /  Disease: Adenoma

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Adenoma
Age 13 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor pahse
Karyotype This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 60% of cells. The rate of polyploidy was 2.4%. Both X and Y chromosomes were normal.
Clinical Data
The tissue donor suffered from Gardner's syndrome, a form of Familial adenomatous polyposis (FAP).
male
Caucasian
13 years
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
The tissue donor suffered from Gardner's syndrome, a form of Familial adenomatous polyposis (FAP). It is an autosomal dominant condition with predisposition to carcinoma and multiple polyps of the colon.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor pahse
Culture Conditions
Temperature: 37.0°C
Name of Depositor EJ Gardner
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation