Detroit 529 (ATCC® CCL-66)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Biosafety Level 1
Age 2.5 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype number = 48; trisomic for X; trisomic for a group G chromosome; Down syndrome
Clinical Data
female
Caucasian
2.5 years
Virus Susceptibility Human poliovirus 1
Comments
The cells have a finite life expectancy of 30 serial passages from the tissue of origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 13,14
D5S818: 12,13
D7S820: 10
THO1: 8,9
TPOX: 8,11
vWA: 18,19
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
Passage History
The cells have a finite life expectancy of 30 serial passages from the tissue of origin.
Year of Origin October, 1963