Channel Catfish Ovary [CCO] (ATCC® CRL-2772)

Organism: Ictalurus punctatus, channel catfish  /  Cell Type: fibroblast  /  Tissue: ovary  / 

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Organism Ictalurus punctatus, channel catfish
Tissue ovary
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age juvenile
Applications CCO is the cell line of choice for the propagation and diagnosis of Channel Catfish Virus (CCV). It is the standard for diagnosing Channel Catfish Virus Disease (CCVD) in farm reared Channel Catfish.
Storage Conditions liquid nitrogen vapor phase
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Derivation
The CCO cell line was derived from the ovaries of a healthy, juvenile channel catfish in October 1975. RefBowser PR, Plumb JA. Fish cell lines: establishment of a line from ovaries of channel catfish. In Vitro 16: 365-368, 1980. PubMed: 7390534
Comments
The cells were cured of mycoplasma contamination in 1989 using Ciprofloxacin [J. Aquat. Anim. Health 6: 82-84, 1994].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 30°C.

Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 30°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%


Name of Depositor L Hanson
Year of Origin October, 1975
References

Bowser PR, Plumb JA. Channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead. J. Wildl. Dis. 16: 451-454, 1980. PubMed: 7411751

Bowser PR, Plumb JA. Fish cell lines: establishment of a line from ovaries of channel catfish. In Vitro 16: 365-368, 1980. PubMed: 7390534

Hanson LA, Thune RL. Ciprofloxacin treatment eliminates Mycoplasma in contaminated channel catfish ovary cells. J. Aquat. Anim. Health 6: 82-84, 1994.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Bowser PR, Plumb JA. Channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead. J. Wildl. Dis. 16: 451-454, 1980. PubMed: 7411751

Bowser PR, Plumb JA. Fish cell lines: establishment of a line from ovaries of channel catfish. In Vitro 16: 365-368, 1980. PubMed: 7390534

Hanson LA, Thune RL. Ciprofloxacin treatment eliminates Mycoplasma in contaminated channel catfish ovary cells. J. Aquat. Anim. Health 6: 82-84, 1994.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm