Pro-5 (ATCC® CRL-1781)

Organism: Cricetulus griseus, hamster, Chinese  / 

Permits and Restrictions

View Restrictions

Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties monolayer or suspension
Biosafety Level 1
Gender female
Applications
The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of the original CHO cell line and is the parental line for several glycosylation mutants (see ATCC CRL-1735, 1736 and 1737).
Clinical Data
female
Comments
This line is a derivative of the original CHO cell line and is the parental line for several glycosylation mutants (see ATCC CRL-1735, 1736 and 1737).
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
Complete Growth Medium Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor P Stanley
References

Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798

Cross References

Nucleotide (GenBank) : U65791 Cricetulus griseus N-acetylglucoaminyltransferase I mRNA, complete cds.

Basic Documentation
Restrictions

The parental Pro-5 line was deposited by Dr. P. Stanley. The cells are not available commercially and it is requested that: 1) In all papers using the cell line a direct reference will be made to Stanley et al. Cell 6:121-128, 1975. 2) Any proposed commercial use of the cells must be negotiated with Dr. P. Stanley, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. 3) No distribution of line derived from the Pro-5 line (including those derived by hybridization and/or transfection) be made to third parties without Dr. Stanley's permission

References

Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798