FAK+/+ (ATCC® CRL-2645)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 8 days gestation
Applications
The FAK+/+ cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene.
It can be used as a control for the FAK-null (ATCC CRL-2644) cell line.
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Expression of the FAK-related protein-tyrosine kinase, Pyk2, is elevated in FAK-/- p53-/- fibroblasts compared with FAK+/+ p53-/- cells.
Derivation
The FAK+/+ cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene. Therefore the cells are genetically FAK+/+ p53-/-.
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Clinical Data
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Oncogene p53 -
Genes Expressed
p53 -
Comments
The FAK+/+ cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene. Therefore the cells are genetically FAK+/+ p53-/-.
It can be used as a control for the FAK-null (ATCC CRL-2644) cell line.
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
Therefore, the cells are genetically FAK-/- p53-/-.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Expression of the FAK-related protein-tyrosine kinase, Pyk2, is elevated in FAK-/- p53-/- fibroblasts compared with FAK+/+ p53-/- cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 12 hrs
Name of Depositor D Ilic
References

Ilic D, et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377: 539-544, 1995. PubMed: 7566154

Sieg DJ, et al. Pyk2 and Src-family protein-tyrosine kinases compensate for the loss of FAK in fibronectin-stimulated signaling events but Pyk2 does not fully function to enhance FAK- cell migration. EMBO J. 17: 5933-5947, 1998. PubMed: 9774338

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ilic D, et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377: 539-544, 1995. PubMed: 7566154

Sieg DJ, et al. Pyk2 and Src-family protein-tyrosine kinases compensate for the loss of FAK in fibronectin-stimulated signaling events but Pyk2 does not fully function to enhance FAK- cell migration. EMBO J. 17: 5933-5947, 1998. PubMed: 9774338