PA317 LXSN (ATCC® CRL-2202)

Organism: Mus musculus, mouse  /  Disease: Leukemia

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain human papilloma viral DNA sequences
Disease Leukemia
Age embryo
Strain NIH/Swiss
Applications
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203).
Derivation
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
Comments
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor DA Galloway
References

Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: The user of the PA317 LXSN cell line agrees to indemnify and hold harmless the United States, the ATCC, Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material.

References

Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902