Schneider's Drosophila Line 2 [D. Mel. (2), SL2] (ATCC® CRL-1963)

Organism: Drosophila melanogaster, fruit fly  /  Tissue: embryo  / 

Organism Drosophila melanogaster, fruit fly
Tissue
embryo
Product Format frozen
Morphology epithelial
Culture Properties suspension or loosely adherent
Biosafety Level 1
Age embryo; 20 to 24 hours
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Images
Comments
The cells have been shown to support the growth of the insect stages of malaria parasites [Pubmed ID: 1734521].
Complete Growth Medium Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.
This medium is formulated for use without CO2.
Subculturing
Protocol: Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Dilute the cell suspension to the desired concentration with fresh medium.
Maintain cultures at a cell concentration between 5 x 104 and 4 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended
Medium Renewal: At the time of subcultivation
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 23.0°C; (Max. 24.0 °C, Min. 22.0°C)
Temperature: 23.0°C
Max Temperature: 24.0°C
Min Temperature: 22.0°C
Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16.0°C and 28.0°C.
Duration: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16C and 28C.
Population Doubling Time approximately 67 hours
Name of Depositor IR Schneider
Year of Origin 1969
References

Warburg A, Miller LH. Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521

Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067

This line was established in 1969 by I. Schneider from several hundred 20 to 24 hour embryos.

Cross References

Nucleotide (GenBank) : AF250759 Drosophila melanogaster hexokinase gene, partial cds.

Basic Documentation
Other Documentation
References

Warburg A, Miller LH. Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521

Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067

This line was established in 1969 by I. Schneider from several hundred 20 to 24 hour embryos.