C3H/MCA clone 16 (ATCC® CRL-1412)

Organism: Mus musculus, mouse  / 

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Strain C3H
Applications
The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene.
Antigen Expression
H-2k
Genes Expressed
H-2k
Tumorigenic Yes
Effects
Yes, in C3H mice
Comments
The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures are prepared using 0.1% trypsin in calcium and magnesium free phosphate buffered saline. New cultures are seeded at 3 X 10 exp3 viable cells/sq cm. Subculture every 6 to 7 days.
Name of Depositor C Heidelberger
References

Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800

Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940

Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242

Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619

Chan GL, Little JB. Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264: 442-444, 1976. PubMed: 1004574

Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800

Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940

Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242

Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619

Chan GL, Little JB. Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264: 442-444, 1976. PubMed: 1004574

Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355