SV-T2 (ATCC® CCL-163.1)

Organism: Mus musculus, mouse  /  Cell Type: fibroblastSV40 transformed  / 

Organism Mus musculus, mouse
Cell Type fibroblastSV40 transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 14 to 17 day gestation embryo
Strain BALB/c
Antigen Expression
H-2d
Genes Expressed
SV40 T-antigen,H-2d
Cellular Products
SV40 T-antigen
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Name of Depositor S Aaronson
References

Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006

Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647

Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416

Basic Documentation
References

Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006

Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647

Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416