NCI-H820 [H820] (ATCC® HTB-181)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: lymph node  /  Disease: papillary adenocarcinoma

Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease papillary adenocarcinoma
Age 53 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Near triploid; modal number = 69; range = 46 to 74; there were 25 to 35 marker chromosomes per metaphase; the i(7p), t(6p;??) and ?i(8q) were the few identifiable markers. One B-like chromosome (Bq+) had a length comparable to N1; another B size chromosome had an interstitial HSR segment. There were 1 to 2 structurally normal X chromosomes, and two or more Y chromosomes were detected in the QM stained metaphases.
Clinical Data
53 years
Caucasian
male
Tumorigenic YES
Effects
in nude mice
Comments

The cell line expresses three surfactant associated proteins (SP-A constitutively, and SP-B and SP-C after dexamethasone induction). 

Electron microscopy shows intracytoplasmic multilamellar bodies suggestive of Type II pneumocytes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 9,11
D7S820: 10,13
THO1: 8
TPOX: 12
vWA: 18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953

The NCI-H820 cell line was derived by A.F. Gazdar and H. Oie in 1984 from the lymph node of a patient with papillary adenocarcinoma of the lung taken prior to treatment.

The cells form typical papillary adenomas.

Basic Documentation
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953

The NCI-H820 cell line was derived by A.F. Gazdar and H. Oie in 1984 from the lymph node of a patient with papillary adenocarcinoma of the lung taken prior to treatment.

The cells form typical papillary adenomas.