NCI-H889 [H889] (ATCC® CRL-5817)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: lymph node  /  Disease: stage E, carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Morphology epithelial
Culture Properties clusters in suspension
Biosafety Level 1
Disease stage E, carcinoma; classic small cell lung cancer
Age 69 years
Gender female
Ethnicity Caucasian
Derivation
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy.
Clinical Data
female
69 years
Caucasian
Comments

The cells express reduced levels of p53 mRNA compared to levels found in normal lung.

There is a point mutation, a G to C transition (TGC to TCC), which results in a substitution of a serine for cysteine at codon 242.

The cells carry the K-ras 12 mutation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Every 2 to 3 days

 

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 8
D5S818: 12
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,9
vWA: 17
Name of Depositor AF Gazdar, JD Minna
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.