Hs 899(D).T (ATCC® CRL-7645)

Organism: Homo sapiens, human  /  Disease: osteosarcoma

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Organism Homo sapiens, human
Product Format flask
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma
Age 20 years
Gender male
Ethnicity Caucasian
Applications
See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.
This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Derivation
See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Clinical Data
See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.
male
Caucasian
20 years
Comments
See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Note: This item is distributed only within the 50 United States. It is not available for international distribution.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Passage History
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation