LL/2 (LLC1) (ATCC® CRL-1642)

Organism: Mus musculus, mouse  /  Tissue: lung  /  Disease: Lewis lung carcinoma

Organism Mus musculus, mouse
Tissue
lung
Product Format frozen
Morphology rounded - loosely attached or floating
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Lewis lung carcinoma
Strain C57BL
Applications
This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Storage Conditions liquid nitrogen vapor phase
Derivation
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Antigen Expression
H-2b
Tumorigenic Yes
Effects
Yes, in C57BL mice
Comments
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Population Doubling Time 21 hrs
Name of Depositor JS Bertram
References

Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207

Basic Documentation
References

Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207