MRC-9 (ATCC® CCL-212)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: lung  /  Disease: normal

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Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 15 weeks gestation
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype normal female; diploid; stable
Clinical Data
15 weeks gestation
Caucasian
female
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17
Name of Depositor JP Jacobs
Year of Origin May, 1974
References

Jacobs JP, et al. Characteristics of a serially propagated human diploid cell designated MRC-9. J. Biol. Stand. 7: 113-122, 1979. PubMed: 113411

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Jacobs JP, et al. Characteristics of a serially propagated human diploid cell designated MRC-9. J. Biol. Stand. 7: 113-122, 1979. PubMed: 113411