CCD-11Lu (ATCC® CCL-202)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 9 years
Gender male
Ethnicity Asian
Applications
The line was derived from normal tissue from patient who died following trauma to the head.
Karyotype normal human male; diploid; stable
Derivation
The line was derived from normal tissue from patient who died following trauma to the head.
Clinical Data
The line was derived from normal tissue from patient who died following trauma to the head.
male
Asian
9 years
Antigen Expression
blood type O; Rh +
Genes Expressed
blood type O; Rh +
Comments
The line was derived from normal tissue from patient who died following trauma to the head.
The cells are capable of at least 12 population doublings beyond the current freeze.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 9,12
D5S818: 11,13
D7S820: 10,12
THO1: 9
TPOX: 8,11
vWA: 18,20
Name of Depositor GP Maxwell