15-79-2 (ATCC® HB-34)

Organism: Mus musculus (pre-B cell); Mus musculus (myeloma), mouse (pre-B cell); mouse (myeloma)  /  Cell Type: hybridoma: pre-B lymphoblast  / 

Organism Mus musculus (pre-B cell); Mus musculus (myeloma), mouse (pre-B cell); mouse (myeloma)
Cell Type hybridoma: pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Age fetus
Applications
The cells produce, but do not secrete mu chain.
No light chain is produced.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells.
No light chain is produced.
Genes Expressed
immunoglobulin; mu heavy chain (not secreted)
Comments
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells.
The cells produce, but do not secrete mu chain.
No light chain is produced.
The kappa light chain genes have not been functionally re-arranged.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Complete Growth Medium
Culture Medium: RPMI medium 1640, 80%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 1 to 2 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor JF Kearney
References

Burrows P, et al. Evidence that murine pre-B cells synthesize mu heavy chains but no light chains. Nature 280: 838-841, 1979. PubMed: 112480

Perry RP, et al. Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. USA 78: 247-251, 1981. PubMed: 6787586

Maki R, et al. Immunoglobulin gene rearrangement in immature B cells. Science 209: 1366-1369, 1980. PubMed: 6774416

Basic Documentation
References

Burrows P, et al. Evidence that murine pre-B cells synthesize mu heavy chains but no light chains. Nature 280: 838-841, 1979. PubMed: 112480

Perry RP, et al. Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. USA 78: 247-251, 1981. PubMed: 6787586

Maki R, et al. Immunoglobulin gene rearrangement in immature B cells. Science 209: 1366-1369, 1980. PubMed: 6774416