SU.86.86 (ATCC® CRL-1837)

Organism: Homo sapiens, human  /  Tissue: pancreas; derived from metastatic site: liver  /  Disease: ductal carcinoma

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Organism Homo sapiens, human
Tissue pancreas; derived from metastatic site: liver
Product Format frozen
Morphology Epithelial
Culture Properties adherent
Biosafety Level 1
Disease ductal carcinoma
Age 57 years
Gender Female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-1837 Micrograph
Derivation

This cell line was developed from a wedge biopsy of a liver metastasis of a pancreatic ductal carcinoma.

Clinical Data
57 years
Caucasian
female
Genes Expressed
carcinoembryonic antigen (CEA)
Tumorigenic Yes
Effects
Yes, in nude mice inoculated subcutaneously with 10(7) cells.
Comments

The cells can be lysed completely in vitro by autologous and allogeneic lymphokine activated killer (LAK) cells in the presence of interleukin 2 (IL-2). They are relatively insensitive to autologous and allogeneic natural killer (NK) cell lysis.

The cells produce CEA, and grow in orthotopic and heterotopic positions in nude mice within 21 days at 100% frequency (5/5).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium. 
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and place at 37°C to facilitate dispersal. Observe cells within 5 to 10 minutes under an inverted microscope 
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 
  6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Recommended seeding densities of 5 x 103 to 4 x 104 viable cells/cm2 
  7. Place culture vessels in incubators at 37°C. Note: subcultures can also be prepared by scraping the cells from the floor of the flask. Add fresh medium, aspirate the scraped cells to disperse them and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended by depositor
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium ,40% ; DMSO,10% ; FBS, 50% FBS..
Storage temperature: liquid nitrogen vapor phase
STR Profile
D5S818: 7,13
D13S317: 11,12
D7S820: 8,11
D16S539: 11,13
vWA: 17
THO1: 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 10,11
Name of Depositor WD Holder
References

Drucker BJ, et al. A new human pancreatic carcinoma cell line produced for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. In Vitro Cell. Dev. Biol. 24: 1179-1187, 1988. PubMed: 3264833

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Drucker BJ, et al. A new human pancreatic carcinoma cell line produced for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. In Vitro Cell. Dev. Biol. 24: 1179-1187, 1988. PubMed: 3264833