clone 9 (ATCC® CRL-1439)

Organism: Rattus norvegicus, rat  /  Cell Type: Epithelial  /  Tissue: liver  /  Disease: normal

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Organism Rattus norvegicus, rat
Tissue liver
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 4 weeks
Gender male
Strain Sprague-Dawley
Applications
This cell line has been used for studies of in vitro carcinogenesis and is useful in clonal assays for screening sera and other nutritional supplements.
Storage Conditions liquid nitrogen vapor phase
Derivation
Clone 9 (K-9) is an epithelial cell line isolated in 1968 from normal liver taken from a young male rat.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Name of Depositor ME Kaighn
    Year of Origin 1968
    References

    . Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975.

    Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    . Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975.

    Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864