PtK2 (NBL-5) (ATCC® CCL-56)

Organism: Potorous tridactylis, potoroo  /  Tissue: kidney  /  Disease: Normal

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Organism Potorous tridactylis, potoroo
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Normal
Age adult
Gender male
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
keratin
Cellular Products
keratin
Virus Susceptibility Human Coxsackievirus A 9
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis New Jersey virus
Virus Resistance
adenovirus 5; coxsackievirus B5; poliovirus 2
Comments

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin 0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37oC to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.  
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6.  Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: growth medium, 95%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor KH Whalen
References

Walen KH. Spatial Relationships in the Replication of Chromosomal DNA. Genetics 51: 915-929, 1965. PubMed: 14337764

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Walen KH. Spatial Relationships in the Replication of Chromosomal DNA. Genetics 51: 915-929, 1965. PubMed: 14337764