CCD 841 CoN (ATCC® CRL-1790™) Organism: Homo sapiens, human / Cell Type: epithelial / Tissue: colon / Disease: normal General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Tissue colon Cell Type epithelial Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease normal Age 21 weeks gestation fetus Gender female Storage Conditions liquid nitrogen vapor phase Karyotype The line is diploid and no consistent marker chromosomes were observed. Clinical Data female fetus 21 weeks gestation Comments Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking. Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°CAtmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile Amelogenin: XCSF1PO: 10,11D13S317: 11,13D16S539: 10,11D5S818: 12,13D7S820: 11THO1: 7,8TPOX: 9,10vWA: 14,18 Name of Depositor A Thompson References . . J. Tissue Culture Methods 9: 117-122, 1985. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References . . J. Tissue Culture Methods 9: 117-122, 1985.