CCD-33Co (ATCC® CRL-1539)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: normal

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Organism Homo sapiens, human
Tissue
colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 7 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This cell line has a trisomic N7 male karyotype, 47,XY,+7 in the majority of cells. However, cells with the normal 46,XY karyotype were also found. The modal chromosome number was 47, occurring in 62% of cells, and cells with 46 chromosome counts also occurred at a high rate at 32%. Apparently this is a mosaic population with the predominant trisomic N7 cells. The rate of polyploidy was 2.0%. Both X and Y chromosomes were present. No other consistent aberrations were detected in all analyzed cells.
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Clinical Data
7 years
Caucasian
male
Comments
Cells have the capacity to proliferate to a maximum of 29 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 11,13
THO1: 6,9.3
TPOX: 9,11
vWA: 15,16
Name of Depositor S Dilworth
Year of Origin June 8, 1979
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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