SNU-C2A (ATCC® CCL-250.1)

Organism: Homo sapiens, human  /  Disease: colorectal carcinoma

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent, multicell aggregates
Biosafety Level 1
Disease colorectal carcinoma
Age 43 years
Gender female
Ethnicity Asian
Applications
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 46; range = 44 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.
Images
Derivation
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
Clinical Data
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
female
Asian
43 years
Antigen Expression
Blood type A; Rh+
Genes Expressed
carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen,Blood type A; Rh+
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).
The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.
Complete Growth Medium ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  1. 0.02 mg/ml insulin
  2. 0.01 mg/ml transferrin
  3. 25 nM sodium selenite
  4. 50 nM Hydrocortisone
  5. 1 ng/ml Epidermal Growth Factor (do not filter)
  6. 0.01 mM ethanolamine
  7. 0.01 mM phosphorylethanolamine
  8. 100 pM triiodothyronine
  9. 0.5% (w/v) bovine serum albumin
  10. 10 mM HEPES
  11. 0.5 mM sodium pyruvate
  12. 2mM L-glutamine(for final conc. of 4.5 mM)
  13. 10% FBS

Subculturing
Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.
Medium Renewal: Two times weekly
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Growth is slow.
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
Passage History
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
Year of Origin 1982
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Basic Documentation
Other Documentation
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells