FDC-P1 (ATCC® CRL-12103)

Organism: Mus musculus, mouse  /  Tissue: bone marrow  /  Disease: Normal

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Tissue bone marrow
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Normal
Strain DBA/2
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor temperature
Karyotype diploid
Derivation
The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).
Antigen Expression
Thy-1.2 (24%)
Comments
They are dependent upon WEHI-3 conditioned medium (WEHI-3CM) for continued growth.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040).
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor University of Virginia Patent Foundation
References

Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334