J27-B7 (ATCC® CRL-2374)

Organism: Mus musculus, mouse  / 

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Strain C3H
Applications
J27-B7 is a stable transfected cell line established in 1987 by transfection with calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65 containing a genomic human B7 gene (gB7).
J27-B7 expresses surface HLA B7 but does not secrete HLA B7.
Derivation
J27-B7 is a stable transfected cell line established in 1987 by transfection with calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65 containing a genomic human B7 gene (gB7).
Antigen Expression
HLA B7
Genes Expressed
HLA B7
Comments
J27-B7 is a stable transfected cell line established in 1987 by transfection with calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65 containing a genomic human B7 gene (gB7).
The cells were selected in HAT and G418.
J27-B7 expresses surface HLA B7 but does not secrete HLA B7.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor F Grumet
Year of Origin 1987
References

Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852